phospho lim kinase 1 thr508  (Bioss)

Journal: Journal of neurochemistry

Article Title: Functional protein kinase arrays reveal inhibition of p-21-activated kinase 4 by alpha-synuclein oligomers.

doi: 10.1111/j.1471-4159.2007.04933.x

Figure Lengend Snippet: Fig. 4 Representative immunoblot analysis of brainstem protein ex- tracts from a a-synuclein (A30P) transgenic (tg) mouse and a wild-type (wt) control animal using antibodies against phospho-LIM kinase 1 and total LIM kinase 1 (LIMK1), respectively. Phosphorylation of LIM ki- nase 1 at Thr508, which becomes phosphorylated by p21-activated kinases, is decreased in transgenic mice. Glyceraldehyde-3-phos- phate dehydrogenase (GAPDH) was assessed as loading control.

Article Snippet: After blocking in buffer containing 20 mmol/L Tris, pH 7.4, 500 mmol/L NaCl, 0.1% Tween 20, and 5% BSA for 90 min, the membranes were incubated overnight with rabbit polyclonal antibodies against phospho-LIM kinase 1 (Thr508) or total LIM kinase 1 (LIMK1) (1 : 1000, Cell Signaling Technology, Beverly, CA, USA) or with rabbit antibodies against full-length a-synuclein (1 : 500, ASY-1, Jensen et al. 2000).

Techniques: Western Blot, Transgenic Assay, Control, Phospho-proteomics